Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells
نویسندگان
چکیده
منابع مشابه
production of recombinant lentiviruses expressing mir-16 by transient transfection of 293t cells
objective: the aim of the present study was the production of recombinant lentviruses that express mir-16. after transduction, altered expression levels of mirna and its target protein were analyzed. methods: a dna fragment that contained the mir-16 precursor was cloned in a lentiviral plasmid. lentiviral vector particles were produced by transient calcium phosphate co-transfection of 293t cell...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2007
ISSN: 1940-087X
DOI: 10.3791/550